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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, happen to be described. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however out there.
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Blocking peptides are peptides that bind specifically for the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope regarded from the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make certain the best possible success are...
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This guide outlines even further probable optimization of mobile and immunoassay parameters to make sure the best possible benefits are...
Antibodies bound to the blocking peptide not bind into the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is certain.
Goal Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress via S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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